Part:BBa_K1943001:Design

fwYellow, yellow chromoprotein reporter system (medium Promoter , weak RBS)

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 172
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 549
  

Design Notes

This biobrick is constructed through 2 steps:

1. Do double enzyme digestion (EcoRI & SpeI for BBa_K1033910, EcoRI & XbaI for BBa_B0015) and do gel extraction, ligation, transformation, picking up single colonies, LB broth incubation, plasmid extraction and gel electrophoresis verification.
2. Do double enzyme digestion (XbaI & PstI for Step 1 product, SpeI & PstI for BBa_K608007) and do gel extraction, ligation, transformation, picking up single colonies, LB broth incubation, plasmid extraction and gel electrophoresis verification.

Source

The following list is the Biobricks we’ve used in construction and their detailed design information.

Part	Type	Discription	Backbone	Location on the plate
BBa_K880005	Promoter + RBS	Strong Promoter + Strong RBS	pSB1C3	3F 2016 Kit Plate 2
BBa_K608007	Promoter + RBS	Medium Promoter + Weak RBS	pSB1C3	5G 2016 Kit Plate 1
BBa_K592012	Chromoprotein	eforRed, red chromoprotein	pSB1C3	15I 2016 Kit Plate 6
BBa_K1033919	Chromoprotein	gfasPurple, purplr chromoprotein	pSB1C3	9K 2016 Kit Plate 6
BBa_K1033910	Chromoprotein	fwYellow, yellow chromoprotein	pSB1C3	6K 2016 Kit Plate 4
BBa_K1033932	Chromoprotein	spisPink, pink chromoprotein	pSB1C3	11K 2016 Kit Plate 6
BBa_B0015	Terminator	Double terminator	pSB1C3	3F 2016 Kit Plate 3

References

1. Parts Registry Assembly Help
2. Parts Registry Transformation Guideline